Er Long Zuo Ci Wan

Deafness Left-Supporting Pill · 耳聾左慈丸

Also known as: Er Ming Zuo Ci Wan (耳鸣左慈丸), Er Ming Wan (耳鸣丸), Chai Ci Di Huang Wan (柴磁地黄丸),

A classical formula used to support hearing and relieve ringing in the ears (tinnitus) caused by a gradual decline in Kidney and Liver Yin. It nourishes the deep reserves of the Kidney, calms rising Liver Yang, and settles the spirit. It is best suited for hearing loss or tinnitus that develops slowly over time, especially in older adults, and is often accompanied by dizziness, blurred vision, and lower back weakness.

Origin 饲鹤亭集方 (Sì Hè Tíng Jí Fāng, Collected Formulas from the Crane-Feeding Pavilion) by Ling Huan (凌奂) — Qīng dynasty, c. 1892 CE
Composition 8 herbs
Shu Di huang
King
Shu Di huang
Ci Shi
King
Ci Shi
Shan Zhu Yu
Deputy
Shan Zhu Yu
Shan Yao
Deputy
Shan Yao
Mu Dan Pi
Assistant
Mu Dan Pi
Fu Ling
Assistant
Fu Ling
Ze Xie
Assistant
Ze Xie
Chai Hu
Envoy
Chai Hu
Explore composition

Educational content Consult qualified TCM practitioners for diagnosis and treatment

Patterns Addressed

In TCM, symptoms don't appear randomly — they cluster into recognizable patterns of disharmony that reveal what's out of balance in the body. Er Long Zuo Ci Wan is designed to correct these specific patterns.

Why Er Long Zuo Ci Wan addresses this pattern

Kidney Yin deficiency is the root pathomechanism this formula targets. When Kidney Yin and essence become depleted, typically through aging, chronic illness, or overwork, the Kidney can no longer nourish the ears (as TCM holds that 'the Kidney opens into the ears'). Insufficient Kidney Yin also fails to anchor Yang, allowing deficiency Heat to rise upward. The formula's core, built around Shu Di Huang with Shan Zhu Yu and Shan Yao, directly replenishes Kidney Yin and essence. Ci Shi further supports the Kidney while settling the rising Yang that Yin deficiency permits. Ze Xie, Fu Ling, and Mu Dan Pi drain the turbidity and deficiency Heat that accumulate when Yin is depleted, ensuring that new Yin is generated in a clean internal environment.

A practitioner would look for one or more of these signs

Tinnitus

Continuous, cicada-like ringing that worsens at night or when fatigued

Hearing Loss

Gradual, progressive hearing decline

Dizziness

Lightheadedness or vertigo

Blurry Vision

Dim or blurry vision

Lower Back Pain

Soreness and weakness in the lower back and knees

Night Sweats

Night sweats or hot palms and soles

Dry Mouth

Dry mouth and throat

Commonly Prescribed For

These conditions can arise from the patterns above. A practitioner would consider Er Long Zuo Ci Wan when these conditions are specifically caused by those patterns — not for all cases of these conditions.

TCM Interpretation

TCM considers the ears to be the sensory opening of the Kidney. A classical teaching from the Ling Shu states that when Kidney Qi reaches the ears, the ears can perceive the five tones. When Kidney Yin and essence gradually decline, whether through aging, chronic illness, or constitutional weakness, insufficient nourishment reaches the ears. At the same time, depleted Yin fails to anchor Yang, and deficiency Heat or Liver Yang can rise along the Shao Yang channel (which passes around the ear) to disturb the ear orifice. This produces a characteristic high-pitched, continuous, cicada-like ringing that tends to worsen at night, with fatigue, and in quiet environments. The tinnitus of Kidney Yin deficiency is typically softer and more persistent than the loud, sudden tinnitus seen with Liver Fire or Phlegm-Fire patterns.

Why Er Long Zuo Ci Wan Helps

Er Long Zuo Ci Wan addresses tinnitus on two levels simultaneously. At the root, Shu Di Huang and Shan Zhu Yu replenish the depleted Kidney Yin and essence that failed to nourish the ears. At the branch, Ci Shi (Magnetite) directly anchors the rising Yang that is disturbing the ear orifice, and is one of the few minerals in the materia medica classically described as 'sharpening hearing.' Chai Hu, in its small envoy dose, opens the Qi pathways of the Shao Yang channel around the ear without being excessively dispersing. Clinical studies have shown Er Long Zuo Ci Wan to achieve an overall effective rate of over 80% for subjective tinnitus when used over a one-month course in patients matching the Yin deficiency pattern.

Also commonly used for

Meniere's Disease

When presenting with Yin deficiency pattern

Hypertension

Hypertension with Liver-Kidney Yin deficiency presentation: dizziness, tinnitus, flushed face

Blurry Vision

Age-related visual decline with concurrent tinnitus and Kidney weakness

Insomnia

Sleep difficulty related to Yin deficiency with Yang rising

What This Formula Does

Every TCM formula has a specific set of actions — here's what Er Long Zuo Ci Wan does in the body, explained in both everyday and TCM terms

Therapeutic focus

In practical terms, Er Long Zuo Ci Wan is primarily used to support these areas of health:

TCM Actions

In TCM terminology, these are the specific therapeutic actions that Er Long Zuo Ci Wan performs to restore balance in the body:

How It Addresses the Root Cause

TCM doesn't just suppress symptoms — it aims to resolve the underlying imbalance. Here's how Er Long Zuo Ci Wan works at the root level.

In TCM, the Kidneys "open into the ears" (肾开窍于耳), meaning that healthy hearing depends on adequate Kidney essence and Yin fluids nourishing the ear. The Liver and Kidneys share a common origin (肝肾同源): the Liver stores Blood while the Kidney stores essence, and together they maintain the Yin foundation of the body. When Kidney Yin becomes depleted, often through aging, chronic overwork, or constitutional weakness, several things go wrong at once.

First, without sufficient Yin to anchor and cool the body's Yang, deficiency Fire flares upward. This produces a characteristic pattern of heat signs in the upper body: flushed cheeks, dry mouth, red tongue with little coating, and a thin, rapid pulse. Second, because the Liver and Kidneys are so closely linked, Kidney Yin deficiency leads to Liver Yin deficiency, which allows Liver Yang to rise unchecked. This ascending, hyperactive Yang disturbs the head and ears, producing tinnitus (often described as a continuous, high-pitched cicada-like sound that worsens at night), progressive hearing loss, dizziness, and blurred vision. The ears, deprived of nourishment from below and disturbed by rising Yang from above, gradually lose their function. This is why the condition typically worsens slowly rather than appearing suddenly.

The formula addresses this by replenishing the depleted Kidney and Liver Yin (treating the root cause), while simultaneously anchoring the rising Yang and opening the ear orifices (treating the symptoms). This dual strategy of tonifying the root and calming the branch is what makes it effective for this particular pattern of hearing decline.

Formula Properties

Every formula has an inherent temperature, taste, and affinity for specific organs — these properties determine how it interacts with the body

Overall Temperature

Slightly Cool

Taste Profile

Predominantly sweet and sour with a salty undertone — sweet to nourish and tonify, sour to astringe and consolidate essence, salty to direct the formula into the Kidneys.

Target Organs

Channels Entered

Kidney Liver

Ingredients

8 herbs

The herbs that make up Er Long Zuo Ci Wan, organized by their role in the prescription

King — Main ingredient driving the formula
Deputy — Assists and enhances the King
Assistant — Supports or moderates other herbs
Envoy — Directs the formula to its target
Kings — Main ingredient driving the formula
Shu Di huang

Shu Di huang

Prepared rehmannia

Dosage 24 - 32g
Temperature Warm
Taste Sweet
Organ Affinity Kidneys, Liver

Role in Er Long Zuo Ci Wan

The chief herb and the foundation of the entire formula. Shu Di Huang is warm, sweet, and enters the Liver and Kidney channels. It powerfully nourishes Kidney Yin, replenishes essence (Jing), and enriches the Blood. When Kidney essence is full, it can ascend to nourish the ears and eyes. Used at the highest dose in the formula, it addresses the root cause of the condition: Kidney Yin deficiency.
Ci Shi

Ci Shi

Magnetite

Dosage 15 - 30g
Temperature Cold
Taste Salty
Organ Affinity Heart, Kidneys, Liver, Lungs
Preparation Calcined (煅磁石); decoct first for 30 minutes (先煎)

Role in Er Long Zuo Ci Wan

The second King herb, specific to this formula's ear-focused indication. Ci Shi is salty and cold, entering the Kidney and Liver channels. It anchors rising Yang, calms the spirit, nourishes the Kidney, and is classically indicated for sharpening hearing and brightening vision. It directly addresses the branch symptom of tinnitus and hearing loss by weighing down the hyperactive Liver Yang that disturbs the ear orifices.
Deputies — Assists and enhances the King
Shan Zhu Yu

Shan Zhu Yu

Cornelian cherries

Dosage 9 - 12g
Temperature Warm
Taste Sour
Organ Affinity Kidneys, Liver

Role in Er Long Zuo Ci Wan

Sour and warm, it supplements and astringes the essence of the Liver and Kidney. It reinforces Shu Di Huang's Yin-nourishing action and prevents the leakage of Kidney essence. By tonifying the Liver, it also helps stabilize Liver Yang.
Shan Yao

Shan Yao

Yam

Dosage 9 - 12g
Temperature Neutral
Taste Sweet
Organ Affinity Kidneys, Lungs, Spleen

Role in Er Long Zuo Ci Wan

Sweet and neutral, it tonifies both the Spleen and the Kidney. It supports the Spleen's role in generating Qi and Blood (which indirectly nourishes the Kidney), while also directly supplementing Kidney Yin. Works with Shan Zhu Yu to strengthen the King herb's replenishing action.
Assistants — Supports or moderates other herbs
Mu Dan Pi

Mu Dan Pi

Mudan peony bark

Dosage 6 - 9g
Temperature Cool
Taste Bitter, Pungent
Organ Affinity Heart, Kidneys, Liver

Role in Er Long Zuo Ci Wan

Bitter, acrid, and slightly cold. It clears Heat, cools the Blood, and invigorates mildly stagnant Blood. In this formula it clears deficiency Fire (especially ministerial Fire from the Liver), and it moderates the warming and cloying nature of Shan Zhu Yu. Paired with Shan Zhu Yu, one tonifies and one drains the Liver.
Fu Ling

Fu Ling

Poria-cocos mushrooms

Dosage 6 - 9g
Temperature Neutral
Taste Sweet
Organ Affinity Heart, Kidneys, Lungs, Spleen

Role in Er Long Zuo Ci Wan

Sweet and bland, it promotes urination, drains Dampness, and strengthens the Spleen. Paired with Shan Yao, it ensures the Spleen can transport and transform properly, preventing the rich, cloying tonics from generating Dampness. It also calms the spirit.
Ze Xie

Ze Xie

Water plantain

Dosage 6 - 9g
Temperature Cold
Taste Sweet
Organ Affinity Urinary Bladder, Kidneys

Role in Er Long Zuo Ci Wan

Sweet and cold, it drains Kidney turbidity and clears deficiency Heat through urination. Paired with Shu Di Huang, it prevents the heavy, cloying nature of the King herb from generating stagnation, and helps drain the floating Fire that rises when Yin is deficient.
Envoy — Directs the formula to its target
Chai Hu

Chai Hu

Bupleurum roots

Dosage 3 - 6g
Temperature Cool
Taste Bitter
Organ Affinity Gallbladder, Liver

Role in Er Long Zuo Ci Wan

Bitter and slightly cold, Chai Hu enters the Liver channel and has a lifting, dispersing quality. In this formula, used in a small dose, it dredges and soothes constrained Liver Qi, helping to smooth the flow of Qi through the Shao Yang channel (which traverses the ear region). It guides the formula's actions toward the Liver and the ears, and assists in calming Liver Yang by resolving underlying Liver Qi constraint.

Why This Combination Works

How the herbs in Er Long Zuo Ci Wan complement each other

Overall strategy

The formula addresses a pattern where Kidney Yin is depleted and can no longer anchor Liver Yang, resulting in hyperactive Yang rising to disturb the ear orifices. The prescription strategy combines rich Yin nourishment at the root with heavy mineral settling to subdue the rising Yang, while small amounts of dispersing herbs ensure Qi flows smoothly through the ear channels.

King herbs

Shu Di Huang (Prepared Rehmannia) is the principal King, used at the highest dose to replenish Kidney Yin and essence. When Kidney essence is sufficient, it naturally ascends to nourish the ears and eyes. Ci Shi (Magnetite) serves as a second King, addressing the branch symptoms directly. Its heavy, sinking mineral nature anchors hyperactive Liver Yang and is classically described as 'sharpening hearing and brightening vision.' Together, these two herbs treat root and branch simultaneously: Shu Di Huang rebuilds what is depleted below while Ci Shi pulls down what has risen inappropriately above.

Deputy herbs

Shan Zhu Yu (Cornus) and Shan Yao (Chinese Yam) reinforce the King herb's nourishing action. Shan Zhu Yu astringes the Liver and Kidney to prevent further leakage of essence, while Shan Yao supports the Spleen, ensuring that the body can properly generate the Qi and Blood needed to sustain Kidney replenishment. Together with Shu Di Huang they form the 'three tonics' (三补) of the Liu Wei Di Huang Wan framework.

Assistant herbs

Mu Dan Pi (Tree Peony Bark) is a restraining Assistant: it clears Liver Fire and deficiency Heat, preventing the warming quality of Shan Zhu Yu from generating excess warmth. Ze Xie (Alisma) is also a restraining Assistant: it drains Kidney turbidity and prevents Shu Di Huang's rich, greasy nature from creating stagnation. Fu Ling (Poria) is a reinforcing Assistant: it drains Dampness and strengthens the Spleen, supporting Shan Yao's digestive function and ensuring the heavy tonifying herbs do not overwhelm the middle. These three 'draining' herbs (三泻) balance the three tonics, creating the elegant open-and-close architecture inherited from Liu Wei Di Huang Wan.

Envoy herbs

Chai Hu (Bupleurum), used in a deliberately small dose, guides the formula to the Liver channel and the Shao Yang region that traverses the ear. Its light, rising, and dispersing nature ensures Qi flows smoothly through the ear passages, counterbalancing the heavy, sinking quality of Ci Shi. A larger dose of Chai Hu would be too dispersing for a Yin-deficient patient; at this small dose, it merely opens the pathway without scattering the precious Yin being built up.

Notable synergies

The Ci Shi and Chai Hu pairing is the hallmark of this formula. Ci Shi sinks and settles while Chai Hu lifts and disperses, creating a balanced dynamic that anchors Yang without trapping Qi in the ear. The classic 'three tonics and three drains' pairing from the parent Liu Wei Di Huang Wan (Shu Di Huang with Ze Xie, Shan Zhu Yu with Mu Dan Pi, Shan Yao with Fu Ling) ensures nourishment without stagnation.

How to Prepare

Traditional preparation instructions for Er Long Zuo Ci Wan

The original text instructs making honey pills (蜜丸). The raw herbs are ground to fine powder and combined with refined honey to form pills. The traditional dose is 9g (approximately 3 qian) per serving, taken twice daily, swallowed with lightly salted warm water (淡盐汤送服). The salt is said to guide the formula downward to the Kidney.

For modern clinical use, the formula can also be prepared as a decoction (水煎服). When converting to decoction form, reduce all herb weights proportionally to standard decoction dosages. Decoct in water and take the resulting liquid in two divided doses daily. Note that Ci Shi (Magnetite) should be decocted first (先煎) for 30 minutes before adding the other herbs, as mineral substances require longer cooking to release their active constituents.

Common Modifications

How practitioners adapt Er Long Zuo Ci Wan for specific situations

Added
Shi Chang Pu

6 - 9g, opens the ear orifices and transforms Phlegm obstructing the sensory passages

Wu Wei Zi

3 - 6g, astringes Kidney essence and quiets the spirit

This modification matches the version recorded in the Chong Ding Guang Wen Re Lun. Shi Chang Pu opens the ear orifice directly, while Wu Wei Zi astringes leaking essence and generates fluids, enhancing the formula's ability to address chronic tinnitus with mental fatigue.

Educational content — always consult a qualified healthcare provider or TCM practitioner before using any herbal formula.

Contraindications

Situations where Er Long Zuo Ci Wan should not be used or requires extra caution

Avoid

Sudden-onset tinnitus or deafness. This formula is only appropriate for gradual hearing decline due to Yin deficiency, not acute conditions which may require urgent medical evaluation.

Avoid

Early stages of acute illness, common cold, or acute infection. Tonifying formulas should not be used when external pathogenic factors are still present.

Avoid

Tinnitus or deafness caused by outer or middle ear pathology (e.g. foreign body in the ear canal, otitis media). These structural causes require conventional medical treatment.

Caution

Phlegm-Dampness obstruction or excessive thick phlegm. The rich, cloying nature of Shu Di Huang and other tonifying herbs may worsen Dampness and impair Spleen function.

Caution

Spleen deficiency with weak digestion, loose stools, or poor appetite. The formula is Yin-nourishing and contains heavy, sticky herbs (Shu Di Huang, Ci Shi) that may burden weak digestion.

Caution

Patients with chronic diseases such as hypertension, heart disease, liver disease, diabetes, or kidney disease should use this formula only under professional guidance.

Special Populations

Important considerations for pregnancy, breastfeeding, and pediatric use

Pregnancy

Not recommended during pregnancy without professional guidance. Mu Dan Pi (Moutan bark) has Blood-moving properties that could theoretically affect pregnancy. Ci Shi (magnetite) is a heavy mineral substance, and Ze Xie has draining properties. While this formula is not strongly contraindicated, pregnant women should consult a qualified practitioner before use. Some commercial preparations explicitly list pregnancy as requiring medical supervision.

Breastfeeding

There is limited specific data on the safety of Er Long Zuo Ci Wan during breastfeeding. The formula consists primarily of gentle tonifying herbs without strongly toxic components. However, the heavy mineral Ci Shi (magnetite) and the draining herbs Ze Xie and Fu Ling could theoretically transfer through breast milk. Breastfeeding mothers should use this formula only under the guidance of a qualified practitioner. Commercial product labels generally advise medical supervision for nursing women.

Children

Er Long Zuo Ci Wan is not commonly indicated for children. The formula addresses Kidney and Liver Yin deficiency patterns that are predominantly seen in middle-aged and elderly adults, particularly age-related hearing decline. Children rarely present with this pattern of gradual Yin depletion. If a practitioner determines it is appropriate for a child, dosage should be significantly reduced (typically one-third to one-half of the adult dose depending on age and body weight), and use should be under close professional supervision. Commercial labels in China generally advise that children use this product only under a physician's guidance.

Drug Interactions

If you are taking pharmaceutical medications, be aware of these potential interactions with Er Long Zuo Ci Wan

Diuretics: Ze Xie (Alisma) and Fu Ling (Poria) both promote urination. Concurrent use with pharmaceutical diuretics (e.g. furosemide, hydrochlorothiazide) could potentially enhance fluid loss and electrolyte imbalance.

Antihypertensive medications: Ci Shi (magnetite) has a Yang-subduing, calming effect and the formula overall can lower blood pressure mildly. Patients on antihypertensives should be monitored for additive blood-pressure-lowering effects.

Aminoglycoside antibiotics (e.g. gentamicin): While research suggests the formula may protect against gentamicin-induced ototoxicity, this interaction has only been studied in animal models. Patients taking ototoxic medications should inform their healthcare provider before using this formula.

Iron and mineral supplements: Ci Shi (magnetite) is an iron-containing mineral. Concurrent use with iron supplements or other mineral preparations may affect absorption of either substance. Separate dosing by at least two hours is advisable.

Usage Guidance

Practical advice for getting the most out of Er Long Zuo Ci Wan

Best time to take

Twice daily, ideally on an empty stomach or 30 minutes before meals, to improve absorption. Traditionally taken with lightly salted water.

Typical duration

Long-term tonic use: typically taken for 1–3 months as an initial course, reassessed by practitioner. May be continued for several months to years for chronic conditions.

Dietary advice

Avoid alcohol, smoking, and spicy, greasy, or heavily fried foods while taking this formula, as these can generate Heat and Dampness that counteract its Yin-nourishing effects. Cold, raw foods and excessive dairy should also be limited, as they may burden the Spleen and impede absorption of the rich tonifying herbs. Favor foods that support Kidney Yin such as black sesame, walnuts, goji berries, mulberries, and dark beans. Light, easy-to-digest meals will help the Spleen transport the formula's nourishing ingredients effectively. The classical instruction to take the pills with lightly salted water was intended to guide the herbs into the Kidney channel.

Er Long Zuo Ci Wan originates from 饲鹤亭集方 (Sì Hè Tíng Jí Fāng, Collected Formulas from the Crane-Feeding Pavilion) by Ling Huan (凌奂) Qīng dynasty, c. 1892 CE

Classical Texts

Key passages from the classical Chinese medical texts that first described Er Long Zuo Ci Wan and its clinical use

《饲鹤亭集方》(Sì Hè Tíng Jí Fāng):
「耳聋左慈丸治肾水不足,虚火上升,头眩目晕,耳聋耳鸣等症。六味加磁石三两,柴胡一两一钱。蜜丸,每服三钱,淡盐汤送。」
"Er Long Zuo Ci Wan treats Kidney Water insufficiency with deficiency Fire flaring upward, manifesting as dizziness, blurred vision, deafness, and tinnitus. [Take] Liu Wei [Di Huang Wan] plus three liang of magnetite and one liang one qian of Chai Hu. Form into honey pills, take three qian per dose, sent down with lightly salted water."

《杂病源流犀烛》(Zá Bìng Yuán Liú Xī Zhú):
「耳鸣者,聋之渐也。」
"Those with tinnitus will gradually become deaf."
This classical observation underscores the progressive nature of Yin-deficiency hearing disorders and the importance of early treatment.

Historical Context

How Er Long Zuo Ci Wan evolved over the centuries — its origins, lineage, and place in the broader tradition of Chinese medicine

Er Long Zuo Ci Wan originates from the Sì Hè Tíng Jí Fāng (《饲鹤亭集方》, "Feihe Pavilion Collection of Prescriptions"), compiled by the Qing Dynasty physician Líng Huàn (凌奂, 1822–1893). Ling Huan, originally from Anji but based in Huzhou (in modern Zhejiang), was a legendary figure in late Qing medicine. He studied under his maternal uncle Wu Gunian, and earned the popular nickname "Ling the Immortal" (凌仙人) for his remarkable clinical skills, including reviving patients thought to be dead. During the Taiping Rebellion era, he treated soldiers' wounds and later organized charitable medical relief during post-war epidemics. His Sì Hè Tíng Jí Fāng collected 450+ formulas across 11 categories and was compiled with the help of his fourth son, Ling Shou.

The formula itself is a modification of Liu Wei Di Huang Wan (Six-Ingredient Rehmannia Pill), the foundational Kidney Yin tonic originally from the Song Dynasty. Ling Huan's innovation was adding just two herbs: Ci Shi (magnetite) to anchor rising Yang and benefit the Kidney and ears, and Chai Hu (bupleurum) to gently raise pure Yang Qi to the ear orifices and soothe the Liver. A later variant recorded in the Chóng Dìng Guǎng Wēn Rè Lùn (《重订广温热论》) substituted Shi Chang Pu (acorus) and Wu Wei Zi (schisandra) for Chai Hu, shifting the formula's emphasis toward opening the orifices and consolidating Kidney essence. The formula was traditionally taken as honey pills sent down with lightly salted water, the salt guiding the medicine into the Kidney channel. Today it is listed in the Chinese Pharmacopoeia and remains one of the most widely used formulas for tinnitus in China.

Modern Research

4 published studies investigating the pharmacological effects or clinical outcomes of Er Long Zuo Ci Wan

1

Effects of Erlong Zuoci Pill and its Disassembled Prescriptions on Gentamicin-Induced Ototoxicity Model In Vitro (In vitro study, 2010)

Dong Y, Cao BY, Wang J, Ding DL, Han ZF, Shi JR. Chinese Journal of Integrative Medicine, 2010, 16(3), 258–263.

This laboratory study tested the formula and its individual herb groups against gentamicin-induced damage to cochlear hair cells in newborn mice. The full formula significantly reduced hair cell loss in a dose-dependent manner. The Liu Wei Di Huang base was found to be the primary protective component, while the addition of magnetite and bupleurum strengthened the overall effect.

DOI
2

Effects of Er-Long-Zuo-Ci-Wan on the Spontaneous Activities of Auditory Central Nucleus in Rat Model of Tinnitus Induced by Salicylate Acid (Animal study, 2009)

Wang YM, Song HY, Tong Z, Qian SJ, Guo RX, Jing ZJ, Shi JR. Zhongguo Ying Yong Sheng Li Xue Za Zhi, 2009, 25(3), 397–401.

In a rat model of chronic tinnitus induced by salicylic acid, Er Long Zuo Ci Wan significantly reduced the abnormally elevated spontaneous neural discharge rates in two key auditory brain regions (the inferior colliculus and secondary auditory cortex), suggesting a mechanism by which the formula may reduce tinnitus perception at the central nervous system level.

PubMed
3

Effects of Erlong Zuoci Decoction on the Age-Related Hearing Loss in C57BL/6J Mice (Animal study, 2016)

Dong Y, Guo CR, Ding Y, Zhang Y, Song HY, Peng YT, Zhang T, Shi JR. Journal of Ethnopharmacology, 2016, 181, 59–65.

This study fed the formula to mice prone to age-related hearing loss over 3 months. Treated mice showed significantly lower auditory brainstem response thresholds (better hearing) compared to untreated controls. The protective mechanism appeared to involve inhibition of p53/Bak-mediated mitochondrial apoptosis in spiral ganglion cells of the cochlea.

DOI
4

Prediction of the Molecular Mechanisms Underlying Erlong Zuoci Treatment of Age-Related Hearing Loss via Network Pharmacology-Based Analyses Combined with Experimental Validation (Network pharmacology + experimental study, 2021)

Liu Q, Li N, Yang Y, Yan X, Dong Y, Peng Y, Shi J. Frontiers in Pharmacology, 2021, 12, 719267.

Using network pharmacology analysis combined with in vivo experiments, this study identified key signaling pathways through which the formula may treat age-related hearing loss, including modulation of cellular senescence, inflammatory responses, and synaptic connections. The findings were validated in a D-galactose-induced aging mouse model.

DOI

Research on TCM formulas is growing but still limited by Western clinical trial standards. These studies provide emerging evidence and should be considered alongside practitioner expertise.