Liang Ge San

Cool the Diaphragm Powder · 凉膈散

A classical formula that clears intense heat from the chest, diaphragm, and digestive tract. It is commonly used for conditions involving high fever, irritability, mouth sores, sore throat, constipation, and a red face or lips caused by excessive internal heat in the upper and middle body. The formula works by both clearing heat from above and moving it downward through the bowels.

Origin Tai Ping Hui Min He Ji Ju Fang (太平惠民和剂局方), Volume 6 — Sòng dynasty, 1107 CE
Composition 8 herbs
Lian Qiao
King
Lian Qiao
Da Huang
Deputy
Da Huang
Mang Xiao
Deputy
Mang Xiao
Huang Qin
Assistant
Huang Qin
Zhi Zi
Assistant
Zhi Zi
Bo He
Assistant
Bo He
Dan Zhu Ye
Assistant
Dan Zhu Ye
Gan Cao
Envoy
Gan Cao
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Educational content Consult qualified TCM practitioners for diagnosis and treatment

Patterns Addressed

In TCM, symptoms don't appear randomly — they cluster into recognizable patterns of disharmony that reveal what's out of balance in the body. Liang Ge San is designed to correct these specific patterns.

Why Liang Ge San addresses this pattern

This is the primary pattern Liang Ge San was designed to treat. When intense heat accumulates in the organs of the upper body (Heart, Lungs) and middle body (Stomach, Intestines), it creates a situation where formless, blazing fire burns upward while formed, dry heat clumps below. Lian Qiao, Huang Qin, Bo He, and Dan Zhu Ye clear the formless fire from above, while Da Huang and Mang Xiao purge the formed accumulation from below. Zhi Zi connects both levels by draining fire through the urine. The formula's strategy of 'purging to clear' is specifically designed for this dual-level heat pattern.

A practitioner would look for one or more of these signs

Breast Heat

Burning sensation in the chest and diaphragm area

Irritability

Restlessness and agitation from heat disturbing the Heart

Sore

Painful sores on the tongue, lips, or mouth lining

Red Face

Flushed red face, dry cracked lips

Thirst

Strong thirst with desire for cold drinks

Constipation

Dry, difficult bowel movements from heat drying the intestines

Dark Urine

Scanty, dark yellow, or burning urination

Sore Throat

Red, swollen, painful throat

Nosebleeds

Epistaxis from heat forcing blood upward

Insomnia

Inability to sleep peacefully due to internal heat

Commonly Prescribed For

These conditions can arise from the patterns above. A practitioner would consider Liang Ge San when these conditions are specifically caused by those patterns — not for all cases of these conditions.

Arises from: Upper and Middle Burner Fire Heart Fire blazing

TCM Interpretation

In TCM, recurrent or severe mouth sores (oral ulceration) are most commonly caused by fire blazing upward from the Heart and Stomach. The Heart opens to the tongue, so Heart fire produces tongue-tip sores and a burning sensation. Stomach heat, meanwhile, travels along the Stomach channel to affect the gums, lips, and inner cheeks. When both organs are affected simultaneously, the entire oral cavity can become inflamed, with widespread painful ulcers, dry cracked lips, foul breath, and thirst. In acute cases, there is often concurrent constipation because the same heat that is rising to the mouth is also drying the intestines.

Why Liang Ge San Helps

Liang Ge San addresses mouth sores through its two-pronged clearing strategy. Lian Qiao and Huang Qin clear the Heart and Lung heat that flares to the mouth and tongue. Da Huang and Mang Xiao purge the Stomach and Intestinal heat that drives fire upward, essentially pulling the source of the fire downward and out through the bowels. Zhi Zi drains heat across all three Burners and conducts fire out through the urine, providing an additional exit route. This bidirectional clearing of fire from above and below is what makes Liang Ge San particularly effective for severe oral inflammation that has not responded to simple heat-clearing herbs alone.

Also commonly used for

Tonsillitis

Acute tonsillitis with swelling, pain, and fever

Viral Conjunctivitis

Red, painful eyes from fire blazing upward

Gingivitis

Gum inflammation, toothache from Stomach fire

Acute Nephritis

Acute icteric hepatitis with upper and middle burner heat

Urinary Tract Infection

Cholecystitis or cholangitis presenting with heat signs

Pneumonia

Lobar pneumonia with high fever and chest heat

Nosebleeds

Epistaxis due to heat forcing blood recklessly upward

What This Formula Does

Every TCM formula has a specific set of actions — here's what Liang Ge San does in the body, explained in both everyday and TCM terms

Therapeutic focus

In practical terms, Liang Ge San is primarily used to support these areas of health:

TCM Actions

In TCM terminology, these are the specific therapeutic actions that Liang Ge San performs to restore balance in the body:

How It Addresses the Root Cause

TCM doesn't just suppress symptoms — it aims to resolve the underlying imbalance. Here's how Liang Ge San works at the root level.

Liang Ge San addresses a condition where intense Heat has accumulated in both the Upper and Middle Burners (the chest, diaphragm, and digestive tract). In TCM theory, the diaphragm area (膈, ge) is a pivotal junction between the upper body (Heart, Lungs) and the middle body (Stomach, intestines). When pathogenic Heat lodges here, it creates a two-level crisis that feeds on itself.

In the Upper Burner, formless (intangible) Fire blazes upward. Because Fire by nature rises, it scorches the sensory openings: the eyes become red, the lips crack and dry, the tongue and throat swell, and mouth sores erupt. When this Heat invades the Heart, it disturbs the spirit (shen), causing restlessness, inability to sleep, and in severe cases, delirious or manic speech. Nosebleeds and vomiting blood can occur as Heat forces Blood out of the vessels.

In the Middle Burner, Heat combines with dry Stomach and Intestinal contents to form tangible, solid obstruction. Fluids are scorched away, so stools become hard and dry, urine turns dark and scanty, and the person feels intensely thirsty. This internal blockage prevents the normal downward movement of Qi through the digestive tract, which paradoxically traps more Heat above, worsening the upper symptoms. The tongue is red with yellow coating and the pulse is slippery and rapid, both confirming intense interior Heat. Clearing only the upper Fire would leave the middle obstruction in place, and purging only the bowels would leave the upper Fire unresolved. Both levels must be addressed simultaneously.

Formula Properties

Every formula has an inherent temperature, taste, and affinity for specific organs — these properties determine how it interacts with the body

Overall Temperature

Cold

Taste Profile

Predominantly bitter and salty-cold, with a sweet moderating note. The bitter flavour drains Heat and dries, the salty flavour softens hardness and purges downward, and the sweet flavour of Gan Cao and honey buffers the harsh purgative action.

Target Organs

Channels Entered

Ingredients

8 herbs

The herbs that make up Liang Ge San, organized by their role in the prescription

King — Main ingredient driving the formula
Deputy — Assists and enhances the King
Assistant — Supports or moderates other herbs
Envoy — Directs the formula to its target
King — Main ingredient driving the formula
Lian Qiao

Lian Qiao

Forsythia fruit

Dosage 12 - 18g
Temperature Slightly Cool
Taste Bitter (苦 kǔ)
Organ Affinity Lungs, Heart, Small Intestine

Role in Liang Ge San

Used at the highest dose to clear Heat and resolve toxicity. Its light, ascending nature allows it to disperse and vent accumulated heat from the Upper Burner and chest, addressing the core pathomechanism of blazing heat in the diaphragm region.
Deputies — Assists and enhances the King
Da Huang

Da Huang

Rhubarb root and rhizome

Dosage 6 - 9g
Temperature Cold
Taste Bitter (苦 kǔ)
Organ Affinity Spleen, Stomach, Large Intestine, Liver, Pericardium
Preparation Wine-washed (酒浸) in the original text

Role in Liang Ge San

Purges heat downward by opening the bowels, clearing accumulated heat and dryness from the Middle Burner and intestines. Together with Mang Xiao, it borrows the Yang Ming (Stomach and Large Intestine) channel as an exit route for heat.
Mang Xiao

Mang Xiao

Mirabilite

Dosage 6 - 9g
Temperature Cold
Taste Bitter (苦 kǔ), Salty (咸 xián)
Organ Affinity Stomach, Large Intestine
Preparation Dissolved into the strained decoction (冲服)

Role in Liang Ge San

Softens hardened stool and flushes accumulated heat and dryness from the intestines. Its salty, cold nature complements Da Huang to clear formed heat clumping in the Middle Burner.
Assistants — Supports or moderates other herbs
Huang Qin

Huang Qin

Baical skullcap root

Dosage 5 - 9g
Temperature Cold
Taste Bitter (苦 kǔ)
Organ Affinity Lungs, Gallbladder, Spleen, Large Intestine, Small Intestine, Heart, Stomach

Role in Liang Ge San

Clears constrained heat from the chest, diaphragm, and Lungs. Focuses its clearing action on the Upper and Middle Burners, reinforcing the King herb's heat-clearing function from a different angle.
Zhi Zi

Zhi Zi

Gardenia fruit

Dosage 5 - 9g
Temperature Cold
Taste Bitter (苦 kǔ)
Organ Affinity Heart, Lungs, San Jiao (Triple Burner)

Role in Liang Ge San

Clears heat from all three Burners and guides fire downward, draining heat through the urine. Connects the upper clearing action with the lower purging action, ensuring fire is conducted out from below.
Bo He

Bo He

Peppermint herb

Dosage 3 - 6g
Temperature Cool
Taste Acrid / Pungent (辛 xīn), Aromatic (芳香 fāng xiāng)
Organ Affinity Lungs, Liver
Preparation Added in the last 5 minutes of decoction (后下)

Role in Liang Ge San

Its light, cool, dispersing nature clears the head, benefits the eyes, and soothes the throat. Vents constrained heat from the Upper Burner outward, embodying the classical principle of 'venting depressed fire' (火郁发之).
Dan Zhu Ye

Dan Zhu Ye

Lophatherum herb

Dosage 3 - 6g
Temperature Cold
Taste Sweet (甘 gān), Bland (淡 dàn)
Organ Affinity Heart, Stomach, Small Intestine

Role in Liang Ge San

Clears heat from the Heart and Upper Burner, promotes urination to guide heat downward and out. Assists Bo He in dispersing upper body heat and calming irritability.
Envoy — Directs the formula to its target
Gan Cao

Gan Cao

Licorice root

Dosage 3 - 6g
Temperature Neutral
Taste Sweet (甘 gān)
Organ Affinity Heart, Lungs, Spleen, Stomach

Role in Liang Ge San

Together with honey, moderates the harsh purging action of Da Huang and Mang Xiao so they clear heat without injuring the Stomach. Its sweet taste 'lingers at the diaphragm' level, protecting fluids and harmonizing all the other herbs.

Why This Combination Works

How the herbs in Liang Ge San complement each other

Overall strategy

The formula simultaneously clears formless heat blazing in the Upper Burner and purges formed dryness-heat accumulating in the Middle Burner. This dual approach, often described as 'purging to clear' (以泻代清), uses the Stomach and Intestines as an exit route for the fire, so that chest and diaphragm heat is resolved from both above and below.

King herbs

Lian Qiao (Forsythia) is used at the heaviest dose in the formula. Its light, cool, dispersing quality makes it especially effective at clearing heat and resolving toxicity in the Upper Burner, particularly around the Heart and chest. It addresses the core problem of accumulated heat trapped in the diaphragm region.

Deputy herbs

Da Huang (Rhubarb) and Mang Xiao (Mirabilite) together form the purgative core, essentially mirroring the action of Tiao Wei Cheng Qi Tang (Regulate the Stomach and Order the Qi Decoction). Da Huang drains excess heat downward through the bowels while Mang Xiao softens any dry accumulation. Together they clear formed heat and dryness from the Middle Burner, providing a downward exit for the fire that has been building up in the chest and diaphragm.

Assistant herbs

Huang Qin (Scutellaria) is a reinforcing assistant that clears constrained heat from the chest and Lungs, supporting the King herb from a complementary angle. Zhi Zi (Gardenia) clears heat across all three Burners and guides fire downward through the urine, bridging the upper-clearing and lower-purging actions. Bo He (Mint) and Dan Zhu Ye (Bamboo Leaf) are light, dispersing assistants that vent heat outward and upward from the head, eyes, and throat, applying the classical principle of 'venting depressed fire.'

Envoy herbs

Gan Cao (Licorice) together with honey serves as the envoy. Their sweet taste moderates the fierce purging power of Da Huang and Mang Xiao, preventing them from draining downward too quickly before the diaphragm heat has been fully cleared. The sweetness also protects Stomach fluids and harmonizes the entire formula.

Notable synergies

The pairing of Lian Qiao with Huang Qin creates a powerful upper-clearing combination that disperses and drains heat from the chest and diaphragm simultaneously. The Da Huang and Mang Xiao pairing with Gan Cao and honey replicates and refines Tiao Wei Cheng Qi Tang, transforming a harsh purgative into a gentler, sustained clearing action. The light ascending herbs (Bo He, Dan Zhu Ye) paired with the heavy descending herbs (Da Huang, Mang Xiao) creates a bidirectional flow that clears heat both upward and downward, leaving the diaphragm cool.

How to Prepare

Traditional preparation instructions for Liang Ge San

In the original source text, all herbs are ground into a coarse powder. For each dose, take 6g of the powder, add 7 pieces of fresh bamboo leaf (Dan Zhu Ye) and a small amount of honey, then decoct in approximately 300ml of water until about 210ml remains. Strain and take warm after meals. For children, the dose is reduced to 1.5g and adjusted according to age. Once the bowels move freely, discontinue the formula.

In modern clinical use, the formula is commonly prepared as a decoction (tang) rather than as a powder. Decoct all herbs except Mang Xiao in water for 20-30 minutes, strain, then dissolve the Mang Xiao and a spoonful of honey into the hot, strained liquid. Bo He (mint) should be added in the last 5 minutes of decoction to preserve its volatile oils. Divide into two doses and take warm.

Common Modifications

How practitioners adapt Liang Ge San for specific situations

Added
Huang Lian

3-6g, to strongly clear Heart fire that causes tongue sores

Huang Lian directly drains Heart fire and clears heat from the Middle Burner, strengthening the formula's ability to resolve painful oral ulceration.

Educational content — always consult a qualified healthcare provider or TCM practitioner before using any herbal formula.

Contraindications

Situations where Liang Ge San should not be used or requires extra caution

Avoid

Pregnancy. This formula contains Da Huang (Rhubarb) and Mang Xiao (Mirabilite), both of which stimulate intestinal motility and can promote uterine contractions. Classical sources explicitly list pregnancy as a contraindication.

Avoid

Patients with constitutional weakness, Qi deficiency, or Spleen-Stomach vacuity. The formula's strongly purgative and cold nature can readily injure Spleen and Stomach Qi, worsening deficiency conditions.

Avoid

Yin deficiency with internal Heat (without true Excess). This formula is designed exclusively for Excess Heat patterns. Using it in Yin-deficient Heat will further damage Yin fluids and deplete the body.

Caution

Elderly or frail patients. The harsh purgative action of Da Huang and Mang Xiao may cause excessive fluid loss, electrolyte imbalance, or collapse in patients with underlying weakness.

Caution

Patients with chronic diarrhea or loose stools. The purgative ingredients will worsen existing bowel looseness and further injure Spleen Qi.

Caution

Once the bowels have been cleared (得利下住服). The original text instructs to stop taking the formula as soon as bowel movements become smooth. Continued use after purgation is achieved risks unnecessary injury to the Stomach and Spleen.

Special Populations

Important considerations for pregnancy, breastfeeding, and pediatric use

Pregnancy

Contraindicated. Da Huang (Rhubarb) and Mang Xiao (Mirabilite/Glauber's salt) are both classified as pregnancy-caution (慎用) or pregnancy-prohibited herbs in classical materia medica. Da Huang promotes intestinal peristalsis and Blood movement, which can stimulate uterine contractions and risk miscarriage. Mang Xiao has strong purgative action that may also endanger pregnancy. The original text of the Tai Ping Hui Min He Ji Ju Fang explicitly notes that pregnant women should avoid this formula (孕妇忌用或慎用本方). This formula should not be used during any trimester of pregnancy.

Breastfeeding

Use with significant caution. Da Huang (Rhubarb) contains anthraquinone compounds that are excreted in breast milk, which may cause diarrhea in nursing infants. Classical materia medica texts note that breastfeeding women should avoid Da Huang for this reason. Mang Xiao's strong purgative effects could also affect the nursing mother's fluid balance and milk production. If this formula is absolutely necessary for a breastfeeding mother, it should be used only briefly under professional supervision, and the infant should be monitored for loose stools or digestive upset.

Children

Liang Ge San has historically been used in pediatrics, particularly for childhood febrile convulsions (小儿惊风) associated with intense Heat. The original Song Dynasty text explicitly includes pediatric dosing instructions: children may take half the adult dose (approximately 1.5g of the powdered formula for young children), adjusted further according to age. However, given the strong purgative action of Da Huang and Mang Xiao, pediatric use requires careful professional supervision. The formula should only be used in children with clear signs of Excess Heat (high fever, constipation, red tongue, yellow coating) and never for deficiency patterns. Once bowel movements normalize, the formula should be stopped immediately to prevent damage to the child's relatively delicate digestive system. Infants and very young children (under age 2) should generally not receive this formula.

Drug Interactions

If you are taking pharmaceutical medications, be aware of these potential interactions with Liang Ge San

Gan Cao (Licorice root) in this formula contains glycyrrhizin, which can cause potassium loss and sodium/water retention with prolonged use. This creates potential interactions with:

  • Cardiac glycosides (e.g. digoxin): Licorice-induced hypokalemia may increase toxicity and risk of arrhythmias.
  • Diuretics (especially thiazides and loop diuretics): Additive potassium depletion, increasing risk of dangerous hypokalemia.
  • Antihypertensive medications: Glycyrrhizin's mineralocorticoid effect (sodium retention, potassium loss) may counteract blood pressure lowering.
  • Corticosteroids: May potentiate both the therapeutic and adverse effects of corticosteroids through similar mineralocorticoid pathways.

Da Huang (Rhubarb) contains anthraquinone glycosides that powerfully stimulate bowel motility. Key interactions include:

  • Oral medications generally: The purgative action may reduce intestinal transit time and decrease absorption of concurrently administered oral drugs.
  • Anticoagulants (e.g. warfarin): Da Huang has mild Blood-moving properties; combined use may theoretically increase bleeding risk.
  • Cardiac glycosides (e.g. digoxin): Diarrhea-induced electrolyte loss (especially potassium) may enhance digoxin toxicity.

Mang Xiao (Mirabilite/sodium sulfate) is an osmotic laxative. The resulting fluid loss and electrolyte shifts can interact with diuretics, antihypertensives, and drugs sensitive to hydration status. Patients on lithium should be cautious, as sodium and fluid changes can alter lithium levels.

Usage Guidance

Practical advice for getting the most out of Liang Ge San

Best time to take

After meals (食后温服), taken warm, as specified in the original text. This helps buffer the strong purgative action on the Stomach.

Typical duration

Acute use only: 1–3 days typically, stopped as soon as bowel movements normalize (得利下住服). Rarely exceeds 5–7 days.

Dietary advice

While taking this formula, avoid greasy, fried, rich, and spicy foods, which generate further Heat and obstruct digestion. Alcohol should be strictly avoided as it adds Heat. Cold, raw foods and iced drinks should also be limited, as although the condition involves Heat, icy foods may impair the Stomach's digestive function during recovery. Light, easily digestible foods are preferred: plain congee (rice porridge), steamed vegetables, and clear soups. Fresh fruits like pears and watermelon, which are naturally cooling, may be taken in moderation. Once the bowels have moved and symptoms improve, gradually return to a normal diet and discontinue the formula promptly.

Liang Ge San originates from Tai Ping Hui Min He Ji Ju Fang (太平惠民和剂局方), Volume 6 Sòng dynasty, 1107 CE

Classical Texts

Key passages from the classical Chinese medical texts that first described Liang Ge San and its clinical use

《太平惠民和剂局方》 (Tai Ping Hui Min He Ji Ju Fang) — Original source text:

Indication: 「凉膈泻热。治上、中二焦积热,烦躁多渴,面热头昏、唇焦咽燥,舌肿喉闭,目赤鼻衄,颔烦结硬,口舌生疮,涕唾稠粘,睡卧不宁,谵语狂妄,大便秘结,小便热赤,以及小儿惊风。」

("Cools the diaphragm and drains Heat. Treats accumulated Heat in the Upper and Middle Burners, with restlessness and excessive thirst, facial flushing and dizziness, dry cracked lips and parched throat, swollen tongue and throat obstruction, red eyes and nosebleeds, hard submandibular swelling, mouth and tongue sores, thick sticky nasal discharge and saliva, restless sleep, delirious and manic speech, constipation, hot dark urine, and also childhood convulsions.")


《医方集解》 (Yi Fang Ji Jie) by Wang Ang:

「此上中二焦泻火药也。热淫于内,治以咸寒,佐以苦甘,故以连翘、黄芩、竹叶、薄荷升散于上,而以大黄、芒消之猛利推荡其中,使上升下行,而膈自清矣;用甘草、生蜜者,病在膈,甘以缓之也。」

("This is a formula for draining Fire from the Upper and Middle Burners. When Heat is excessive in the interior, treat with salty-cold, assisted by bitter-sweet. Therefore, Lian Qiao, Huang Qin, Zhu Ye, and Bo He scatter and disperse upward, while Da Huang and Mang Xiao forcefully scour the middle, making what is above ascend and what is below descend, and the diaphragm clears naturally. Gan Cao and raw honey are used because the disease is located at the diaphragm, and sweet flavour slows [the formula's action to keep it there].")


《成方便读》 (Cheng Fang Bian Du):

「故以大黄、芒硝之荡涤下行者,去其结而逐其热……连翘解散经络中之余火;栀子自上而下,引火邪屈曲下行……用甘草、生蜜者,病在膈,甘以缓之也。」

("Therefore Da Huang and Mang Xiao scour downward, removing the clumping and expelling the Heat… Lian Qiao disperses residual Fire from the channels; Zhi Zi guides Fire pathogen downward in a winding path… Gan Cao and raw honey are used because the disease is in the diaphragm, and sweet flavour slows the formula's action.")

Historical Context

How Liang Ge San evolved over the centuries — its origins, lineage, and place in the broader tradition of Chinese medicine

Liang Ge San first appeared in the Tai Ping Hui Min He Ji Ju Fang (太平惠民和剂局方), the official government pharmacopoeia compiled during the Song Dynasty and published around 1107 CE. This text was essentially a state-sponsored formulary, designed to standardize prescriptions dispensed by government pharmacies across the empire. Liang Ge San was one of its most celebrated formulas for treating intense internal Heat conditions.

Later commentators recognized that Liang Ge San is essentially an expansion of Zhang Zhongjing's Tiao Wei Cheng Qi Tang (Regulate the Stomach and Order the Qi Decoction) from the Shang Han Lun, which also uses Da Huang, Mang Xiao, and Zhi Gan Cao. The Song-dynasty formulators added Lian Qiao, Huang Qin, Zhi Zi, Bo He, Zhu Ye, and honey to address Heat in the Upper Burner simultaneously with the Middle Burner purgation. As the Qing-dynasty physician Jiao Shude noted, this shows how many "modern formulas" (时方) evolved directly from classical prescriptions (经方).

During the Qing Dynasty, Warm Disease (温病) physicians adopted Liang Ge San as a key formula for febrile epidemics. Ye Tianshi used it in his Lin Zheng Zhi Nan Yi An for severe Heat reversal cases, while other practitioners modified it for epidemic rashes and throat conditions. Yu Shixiong, in his Yi Zhen Yi De, famously replaced Da Huang and Mang Xiao with Shi Gao (Gypsum) for treating epidemic rashes, reasoning that purgatives were too harsh for the formless toxin of eruptive disease. In modern times, the formula has attracted pharmacological research interest for its anti-inflammatory properties, particularly in acute lung injury and sepsis models.

Modern Research

3 published studies investigating the pharmacological effects or clinical outcomes of Liang Ge San

1

LGS protects against LPS-induced inflammation through cholinergic anti-inflammatory pathway (Preclinical in vitro/in vivo study, 2016)

Liu JS, Wei XD, Lu ZB, Xie P, Zhou HL, Chen YY, Ma JM, Yu LZ. Oncotarget, 2016, 7(16): 21222-21234.

This study found that Liang Ge San significantly suppressed the production of inflammatory cytokines IL-6 and TNF-alpha in LPS-stimulated macrophages. The formula activated the alpha-7 nicotinic acetylcholine receptor and inhibited the NF-kB signaling pathway. In a rat model of acute lung injury, LGS reduced lung inflammation through this cholinergic anti-inflammatory mechanism.

DOI
2

LGS attenuates LPS-induced acute lung injury through up-regulating miR-21 (Preclinical study, 2020)

Yang H, Liu JS, Yan LJ, et al. Frontiers in Pharmacology, 2020, 10: 1574.

This study demonstrated that Liang Ge San exerted anti-inflammatory effects in LPS-induced acute lung injury by upregulating microRNA-21 expression, which in turn inhibited the STAT3 signaling pathway and reduced production of inflammatory cytokines IL-6, TNF-alpha, and IL-1beta in both cell culture and animal models.

PubMed
3

LGS attenuates acute inflammation via targeting GSK3β (Preclinical bioinformatics and in vitro/in vivo study, 2023)

Yang L, Yan L, Tan W, Zhou X, Yang G, Yu J, Lu Z, Liu Y, Zou L, Li W, Yu L. Frontiers in Pharmacology, 2023, 14: 1181319.

Using bioinformatics analysis of sepsis gene datasets combined with network pharmacology, this study identified glycogen synthase kinase-3beta (GSK-3beta) as a key therapeutic target of Liang Ge San in acute inflammation. Cell and zebrafish experiments confirmed that LGS inhibited GSK-3beta activity and reduced inflammatory markers, supporting its potential role in sepsis treatment.

DOI

Research on TCM formulas is growing but still limited by Western clinical trial standards. These studies provide emerging evidence and should be considered alongside practitioner expertise.